Genetic determinants of a nickel-specific transport system are part of the plasmid-encoded hydrogenase gene cluster in Alcaligenes eutrophus

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:7164635

Eberz, G; Eitinger, T; Friedrich, B ^[1]

---

1. Freien Universitaet Berlin (Germany, F.R.)

Nickel-deficient Nic{sup {minus}} mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterizied. The Nic{sup {minus}} mutants carried defined deletions in the hydrogenase gene cluster of the indigenous pHG megaplasmid. Nickel deficiency correlated with a low level of the nickel-containing hydrogenase activity, a slow rate of nickel transport, and reduced activity of urease. The Nic{sup +} phenotype was restored by a cloned DNA sequence (hoxN) of a megaplasmid pHG1 DNA library of A. eutrophus H16. hoxN is part of the hydrogenase gene cluster. The nickel requirement of Nic{sup {minus}} mutants was enhanced by increasing the concentrations of magnesium. This suggests that the Nic{sup {minus}} mutants are impaired in the nickel-specific transport system and thus depend on the second transport activity which normally mediates the uptake of magnesium.

• OSTI ID: 7164635
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:3; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METALS
BACTERIA
CELL CONSTITUENTS
CLONING
DNA HYBRIDIZATION
DNA-CLONING
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GENES
GROWTH
HYBRIDIZATION
HYDROGENASES
MAGNESIUM
METABOLISM
METALS
MICROORGANISMS
MUTANTS
NICKEL
OXIDOREDUCTASES
PHENOTYPE
PLASMIDS
TRANSITION ELEMENTS
UPTAKE