High-level nickel resistance in Alcaligenes xylosoxydans 31A and Alcaligenes eutrophus KTO2

Journal Article · Fri Nov 01 04:00:00 EST 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5878068

Schmidt, T; Stoppel, R D; Schlegel, H G ^[1]

---

1. Inst. fuer Mikrobiologie der Georg-August-Univ., Goettingen (West Germany)

Two new nickel-resistant strains of Alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. Alcaligenes xylosoxydans 31A is a heterotrophic bacterium, and Alcaligenes eutrophus KTO2 is an autotrophic aerobic hydrogen-oxidizing bacterium. Both strains carry - among other plasmids - a megaplasmid determining resistance to 20 to 50 mM NiCl{sub 2} and 20 mM CoCl{sub 2} (when growing in defined Tris-buffered media). Megaplasmid pTOM8, pTOM9 from strain 31A, and pGOE2 from strain KTO2 confer nickel resistance to the same degree to transconjugants of all strains of A. eutrophus tested but were not transferred to Escherichia coli. However, DNA fragments carrying the nickel resistance genes, cloned into broad-host- range vector pVDZ{prime}2, confer resistance to A. eutrophus derivatives as well as E. coli. The DNA fragments of both bacteria, TBA8, TBA9, and GBA (14.5-kb BamHI fragments), appear to be identical. They share equal size, restriction maps, and strong DNA homology but are largely different from fragment HKI of nickel-cobalt resistance plasmid pMOL28 of A. eutrophus CH34.

• OSTI ID: 5878068
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 57:11; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540220 -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
CELL CONSTITUENTS
CLONING
COBALT
DNA
DNA HYBRIDIZATION
DNA-CLONING
ELEMENTS
GENES
HYBRIDIZATION
METALS
MICROORGANISMS
MOLECULAR BIOLOGY
NICKEL
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLASMIDS
SENSITIVITY
TOLERANCE
TOXICITY
TRANSITION ELEMENTS