Cloning of plasmid genes encoding resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus CH34

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · J. Bacteriol.; (United States)

OSTI ID:5313058

Nies, D; Mergeay, M; Friedrich, B; Schlegel, H G

A 238-kilobase-pair plasmid, pMOL30, confers resistance to cadmium, zinc, and cobalt salts in Alcaligenes eutrophus CH34. After Tn5 mutagenesis, restriction nuclease analysis, and Southern DNA-DNA hybridization, a 9.1-kilobase-pair EcoRI fragment was found to harbor all of these resistance properties and was cloned into the broad-host-range hybrid plasmid pRK290. When transferred to a plasmid-free derivative of CH34, the hybrid plasmid conferred the same degree of resistance as the parent plasmid pMOL30. In two other Alcaligenes strains, the hybrid plasmid was expressed, but to a lower degree than in CH34 derivatives.

• Research Organization: Freien Universitaet Berlin (West Germany)
• OSTI ID: 5313058
• Journal Information: J. Bacteriol.; (United States), Vol. 169:10
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
SENSITIVITY
CADMIUM
MUTAGENESIS
COBALT
NUCLEASES
DNA-CLONING
ZINC
BIOLOGICAL MARKERS
HYBRIDIZATION
PLASMIDS
RECOMBINANT DNA
CELL CONSTITUENTS
CLONING
DNA
ELEMENTS
ENZYMES
ESTERASES
HYDROLASES
METALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
TRANSITION ELEMENTS
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry