The use of protein adducts of benzo(a)pyrene for exposure assessment
- Environmental Protection Agency, Cincinnati, OH (USA)
The present study was designed to quantitate B(a) adducts formed not only with globin but also with other blood proteins (albumin, {gamma}-globulins and red cell membrane) and correlate with the level of B(a)P-DNA adducts formed in liver, lung, stomach and kidney. Benzo(a)pyrene 30 mg/kg (or) 1 mg/mouse, containing 300 {mu}Ci of (H{sup 3}) BP in 0.2 ml dimethylsulfoxide, was administered orally to 10 to 12 week old male B{sub 6}C{sub 3}F{sub 1} mice and sacrificed after 8, 24, 36, 48 and 96 hours, at which time the B(a)P bound to blood proteins and tissue DNA was determined. The levels of protein and DNA adducts of B(a)P metabolites are presented. The adduct formation with albumin, {gamma}-globulin and globin appears to reach a maximum by 24 hours and showed a subsequent decline throughout the study period. On the other hand, the binding with red cell membranes reached its maximum by 8h and showed a steady decline up to 96h; whereas the B(a)P-DNA adducts formed in the liver, kidney and lung reached the maximum between 36-48h. The DNA adducts in the stomach reached its optimum level by 24h. The level of DNA binding following oral administration was shown to be highest in the stomach, followed by liver, lung and kidney.
- OSTI ID:
- 7164552
- Report Number(s):
- CONF-8909236--
- Journal Information:
- Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA), Journal Name: Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA) Vol. 28:2; ISSN 0099-7293; ISSN ACWCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ALBUMINS
AROMATICS
BENZOPYRENE
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONDENSED AROMATICS
DISTRIBUTION
DNA ADDUCTS
ERYTHROCYTES
GLOBIN
GLOBULINS
HYDROCARBONS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MATERIALS
MEMBRANE PROTEINS
METABOLISM
ORGANIC COMPOUNDS
PROTEINS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS