Cellular sites of estrogen and antiestrogen uptake, retention and action: comparative autoradiographic studies in the immature rat uterus

Thesis/Dissertation · Wed Dec 31 23:00:00 EST 1986

OSTI ID:7164345

Ennis, B W

This purpose of this study is to clarify the mechanism of action of antiestrogens: agents used for treating breast cancer and as probes for studying the mechanisms of action of estrogen. Nuclear uptake and retention of estrogen and antiestrogen were determined in the different cell types of the immature rat uterus, by quantitative autoradiography, after an injection of tritiated hydroxytamoxifen ((/sup 3/H)TAM(OH)) or tritiated estradiol ((/sup 3/H)E/sub 2/). The effect of TAM(OH) and E/sub 2/ on progesterone receptor content was assessed in the different cell types by determining nuclear uptake of the synthetic progestin (/sup 3/H)Org 2058. The results indicate that antiestrogen and estrogen localize to nuclei of the same uterine cell types, but that this nuclear uptake differs among the uterine tissue compartments, that antiestrogen is taken up considerably slower and retained longer than estrogen and that antiestrogen and estrogen differentially affect progesterone receptor content in the different cell types. The results further suggest that antiestrogen-specific binding sites exist in the cytoplasm of uterine luminal epithelium.

• Research Organization: North Carolina Univ., Chapel Hill (USA)
• OSTI ID: 7164345
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BODY
COMPARATIVE EVALUATIONS
EPITHELIUM
ESTROGENS
FEMALE GENITALS
HORMONES
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROGESTERONE
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RETENTION
RODENTS
STEROID HORMONES
STEROIDS
TISSUES
TRITIUM COMPOUNDS
UPTAKE
UTERUS
VERTEBRATES