Differential induction of progestin-binding sites in uterine cell types by estrogen and antiestrogen

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-123-4-1747· OSTI ID:6736845

Ennis, B W; Stumpf, W E

Effects of antiestrogen on progestin binding in uterine cell types were determined and compared to those of estrogen. Effects on uterine morphology were also studied. Immature rats were treated with four daily sc injections of 100 micrograms hydroxytamoxifen (TAM(OH)), 5 micrograms estradiol (E2), or oil. On day 5 the rats were injected iv with 1 microgram of the synthetic progestin (/sup 3/H)Org 2058, and 1 h later uteri were excised, weighed, and processed for thaw-mount autoradiography. Treatment with TAM(OH) or E2 resulted in uterine weight gain, which was greater in animals treated with E2. E2 treatment resulted in cellular hypertrophy in all tissue compartments, especially in the luminal epithelium and myometrium, but TAM(OH) treatment resulted in hypertrophy of only the luminal epithelium. Treatment with TAM(OH) or E2 changed the pattern and intensity of nuclear binding of (/sup 3/H)Org 2058 from that in oil-treated controls. E2 increased progestin binding in stroma and myometrium and decreased it in luminal epithelium. TAM(OH), similarly, decreased progestin binding in the luminal epithelium and increased it, albeit less than E2, in the myometrium, but left it unchanged in the stroma. The results indicate that E2 and TAM(OH) differentially effect progestin binding among the uterine tissue compartments.

• Research Organization: Univ. of North Carolina, Chapel Hill (USA)
• OSTI ID: 6736845
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 123:4; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BODY
EPITHELIUM
ESTRADIOL
ESTRANES
ESTROGENS
FEMALE GENITALS
HORMONES
HYDROXY COMPOUNDS
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MORPHOLOGY
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PREGNANES
PROGESTERONE
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
STEROID HORMONES
STEROIDS
TAMOXIFEN
TISSUES
TRITIUM COMPOUNDS
UTERUS
VERTEBRATES