In vitro metabolism of t-2 mycotoxin 1,2. Interim report

Technical Report · Mon Nov 10 04:00:00 EST 1986

OSTI ID:7162201

Thompson, W L; Pace, J G; O'Brien, J C

In vitro metabolism of T-2 mycotoxin (T-2) was studied in Vero cells, rat spleen lymphocytes, chicken embryo heart cells, rat small intestinal segments, and rat liver hepatocytes. The method used was thin-layer chromatography (TLC) of (3H)T-2 and its metabolic products, followed by radioactive scanning of the plates. Vero cells, lymphocytes, and heart cells metabolized 5 to 35% of the T-2 to HT-2 mycotoxin (HT-2) after 24-hr exposure. No other metabolites were detected with these three cell systems. Rat intestinal segments, everted onto pipets, converted T-2 into three metabolites migrating in the range between T-2 tetraol and HT-2 on the TLC plates. Hepatocytes metabolized T-2 most rapidly, as indicated by complete disappearance of the parent compound within 4 hr. In addition to the T-2 peak, four predominant peaks appeared on the plates, one of them, increasing with time at the origin, was predominantly composed of glucuronide conjugates.

• Research Organization: Army Medical Research Inst. of Infectious Diseases, Fort Detrick, MD (USA)
• OSTI ID: 7162201
• Report Number(s): AD-A-192647/6/XAB
• Country of Publication: United States
• Language: English

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