Determination of the functional size of oxytocin receptors in plasma membranes from mammary gland and uterine myometrium of the rat by radiation inactivation
Gel filtration of detergent-solubilized oxytocin (OT) receptors in plasma membrane fractions from both regressed mammary gland and labor myometrium of the rat, showed that specific (/sup 3/H)OT binding was associated with a heterogeneously sized population of macromolecules. As radiation inactivation is the only method available to measure the apparent molecular weights of membrane proteins in situ, we used this approach to define the functional sizes of OT receptors. The results indicate that both mammary and myometrial receptors are uniform in size and of similar molecular mass. Mammary and myometrial receptors were estimated to be 57.5 +/- 3.8 (SD) and 58.8 +/- 1.6 kilodaltons, respectively. Knowledge of the functional size of OT receptors will be useful in studies involving the purification and characterization of the receptor and associated membrane components.
- Research Organization:
- Medical College of Ohio, Toledo (USA)
- OSTI ID:
- 7154978
- Journal Information:
- Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 122:5; ISSN ENDOA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560152 -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
FEMALE GENITALS
GLANDS
HORMONES
IRRADIATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MAMMARY GLANDS
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
ORGANS
OXYTOCIN
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SIZE
SOLUBILITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
VERTEBRATES