Arginine metabolism in wounds

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Am. J. Physiol.; (United States)

OSTI ID:7154379

Albina, J E; Mills, C D; Barbul, A; Thirkill, C E; Henry, Jr, W L; Mastrofrancesco, B; Caldwell, M D

Arginine metabolism in wounds was investigated in the rat in 1) lambda-carrageenan-wounded skeletal muscle, 2) Schilling chambers, and 3) subcutaneous polyvinyl alcohol sponges. All showed decreased arginine and elevated ornithine contents and high arginase activity. Arginase could be brought to the wound by macrophages, which were found to contain arginase activity. However, arginase was expressed by macrophages only after cell lysis and no arginase was released by viable macrophages in vitro. Thus the extracellular arginase of wounds may derive from dead macrophages within the injured tissue. Wound and peritoneal macrophages exhibited arginase deiminase activity as demonstrated by the conversion of (guanido-/sup 14/C)arginine to radiolabeled citrulline during culture, the inhibition of this reaction by formamidinium acetate, and the lack of prokaryotic contamination of the cultures. These findings and the known metabolic fates of the products of arginase and arginine deiminase in the cellular populations of the wound suggest the possibility of cooperativity among cells for the production of substrates for collagen synthesis.

• Research Organization: Rhode Island Hospital, Providence (USA)
• OSTI ID: 7154379
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 254; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDASES
AMIDES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ARGINASE
ARGININE
BIOLOGICAL RECOVERY
CARBON 14 COMPOUNDS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CITRULLINE
COLLAGEN
CONNECTIVE TISSUE CELLS
ENZYME ACTIVITY
ENZYMES
HEALING
HYDROLASES
INJURIES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MACROPHAGES
MAMMALS
METABOLISM
MUSCLES
NON-PEPTIDE C-N HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTES
PROTEINS
RATS
RECOVERY
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
TRACER TECHNIQUES
UREA
VERTEBRATES
WOUNDS