Stimulation by D-glucose of the direct conversion of arginine to citrulline in enterocytes isolated from pig jejunum

Journal Article · Fri Jun 28 04:00:00 EDT 1991 · Biochemical and Biophysical Research Communications; (United States)

DOI:https://doi.org/10.1016/0006-291X(91)90663-R· OSTI ID:5316961

Blachier, F; M'Rabet-Touil, H; Darcy-Vrillon, B; Posho, L; Duee, P H ^[1]

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1. Inst. National de la Recherche Agronomique, JOUY-en-JOSAS (France)

In enterocytes isolated from pig jejunum, L-arginine is metabolized to L-citrulline either directly or indirectly through the sequence of reactions catalysed by arginase and ornithine transcarbamylase. In the presence of 5 mM D-glucose, the direct conversion of 1mM L-(guanido-14C) arginine to L-citrulline was increased more than 4 times. Isolated enterocytes exhibit a high glycolytic capacity. Furthermore, the decarboxylation of 5mM D-(1-14C) glucose was 3.6 fold higher than the decarboxylation of 5 mM D-(6-14C) glucose which suggests the presence of a pentose phosphate pathway in enterocytes. Since the production of labelled L-citrulline from L-(guanido-14C) arginine in pig enterocyte homogenates was markedly increased in the presence of NADPH, it is proposed that the direct conversion of L-arginine to L-citrulline could be stimulated by the production of NADPH from D-glucose in the pentose phosphate pathway.

• OSTI ID: 5316961
• Journal Information: Biochemical and Biophysical Research Communications; (United States), Journal Name: Biochemical and Biophysical Research Communications; (United States) Vol. 177:3; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMIDASES
AMIDES
AMINO ACIDS
AMYLASE
ANIMALS
ARGINASE
ARGININE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CITRULLINE
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ENZYMES
GASTROINTESTINAL TRACT
GLUCOSE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
IN VITRO
INTESTINES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NON-PEPTIDE C-N HYDROLASES
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
REACTION KINETICS
SACCHARIDES
SMALL INTESTINE
SWINE
SYNTHESIS
TRACER TECHNIQUES
UREA
VERTEBRATES