Molecular characterization of Pseudomonas aeruginosa bacteriophages: identification and characterization of the novel virus B86

Journal Article · Sun May 01 04:00:00 EDT 1988 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(88)90636-8· OSTI ID:7152331

Kilbane, J J; Miller, R V

We have characterized a new phage, B86, of Pseudomonas aeruginosa isolated from nature. It is a temperate, uv-inducible, generalized transducing phage. To determine the relatedness of this phage to other characterized P. aeruginosa phages, DNA homology studies were carried out. P. aeruginosa phages have previously been grouped by immunological cross-reactivity. Our studies confirm this classification by demonstrating that phages of different class share little or no DNA homology. Based on homology studies as well as cross-immunity to superinfection, B86 is related to other class B phages and is most homologous with phage B39. The virion morphology of these two phages is quite different, however, as are the restriction enzyme digestion patterns of their genomes with several restriction enzymes. Wild-type B86 is subject to the host-controlled restriction-modification systems of P. aeruginosa PAO and PAT. Virulent mutants of this phage are not restricted by these same restriction-modification systems.

• Research Organization: Loyola Univ. of Chicago, Maywood, IL (USA)
• OSTI ID: 7152331
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 164:1; ISSN VIRLA
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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BACTERIOPHAGES
CHEMICAL COMPOSITION
DNA
ELECTROMAGNETIC RADIATION
IMMUNE REACTIONS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PSEUDOMONAS
RADIATIONS
ULTRAVIOLET RADIATION
VIRUSES