Two loci in the genome of transposable phage B39 of Pseudomonas aeruginosa affecting integration. II. Mapping of the pdeX and pdeY loci by restriction and heteroduplex analyses

Journal Article · Wed Oct 01 04:00:00 EDT 1986 · Sov. Genet. (Engl. Transl.); (United States)

OSTI ID:5783293

Akhverdyan, V Z; Kirsanov, N B; Khrenova, E A; Cheremukhina, L V; Rebentish, B A; Krylov, V N

Two loci termed pdeX and pdeY have previously been identified in the genome of transposable phage B39 of Pseudomonas aeruginosa. Both of these loci must operate concomitantly for the normal expression of the phage. In the present study the pdeX (2.85-6.5 kb) and pdeY (6.4-16 kb) loci have been mapped by means of restriction and heteroduplex analysis of hybrid phages possessing the Pde/sup +/ and Pde/sup -/ phenotypes and obtained from the transposable phage B39ts/sup +/ x PH132 cross. The EcoRV-sites in genomes of phages D3112, B39, B39ts/sup +/ and PH132 have been mapped and the previously undetermined EcoRI-sites in the phage B39 genome have also been mapped. The Kpn1 site in the phage Ph132 genome has been localized.

• OSTI ID: 5783293
• Journal Information: Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 22:4; ISSN SOGEB
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ELECTROPHORESIS
ENZYMES
ESTERASES
GENES
GENETIC MAPPING
GENETICS
HYBRIDIZATION
HYDROLASES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LYSIS
MAPPING
MICROORGANISMS
NUCLEASES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PSEUDOMONAS
RADIOENZYMATIC ASSAY
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VIRUSES