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Expression of Pseudomonas aeruginosa transposable phages in Pseudomonas putida cells. I. Establishment of lysogeny and lytic growth efficiency

Journal Article · · Sov. Genet. (Engl. Transl.); (United States)
OSTI ID:5652969
; ; ; ;

Expression of the genomes of Pseudomonas aeruginosa transposable phages (TP) in the cells of a heterologous host, P. putida PpGl, was studied. A high efficiency of TP lytic growth in PpGl cells was obtained both after zygotic induction following RP4::TP plasmid transfer and after thermoinduction of PpGl cells lysogenic for thermoinducible prophage D3112cts15. Characteristic for PpGl cells was a high TP yield (20-25 phage D3112cts15 particles per cell), which was evidence of a high level of TP transposition in cells of this species. The frequency of RP4::TP transfer into PpGl and PA01 cells was equal, but the lysogeny detection rat was somewhat lower in PpGl. Pseudomonas aeruginosa TP can integrate into the PpGl chromosome, producing inducible lysogens. The presence of RP4 is not necessary for the expression of the TP genome in PpGl cells. The D3112cts15 TP may be used for interspecific transduction of plasmids and chromosomal markers.

Research Organization:
All-Union Scientific-Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, USSR
OSTI ID:
5652969
Journal Information:
Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 21:9; ISSN SOGEB
Country of Publication:
United States
Language:
English

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Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGY
CELL CONSTITUENTS
CELL CULTURES
CHEMISTRY
CHROMOSOMES
CLONE CELLS
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
DNA REPLICATION
EFFICIENCY
GENES
GENETICS
GROWTH
HYBRIDIZATION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LYSIS
MICROORGANISMS
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PSEUDOMONAS
RADIOISOTOPES
REACTION KINETICS
TEMPERATURE EFFECTS
TRACER TECHNIQUES
TRANSCRIPTION
VIRUSES