Expression of Pseudomonas aeruginosa transposable phages in Pseudomonas putida cells. I. Establishment of lysogeny and lytic growth efficiency
Expression of the genomes of Pseudomonas aeruginosa transposable phages (TP) in the cells of a heterologous host, P. putida PpGl, was studied. A high efficiency of TP lytic growth in PpGl cells was obtained both after zygotic induction following RP4::TP plasmid transfer and after thermoinduction of PpGl cells lysogenic for thermoinducible prophage D3112cts15. Characteristic for PpGl cells was a high TP yield (20-25 phage D3112cts15 particles per cell), which was evidence of a high level of TP transposition in cells of this species. The frequency of RP4::TP transfer into PpGl and PA01 cells was equal, but the lysogeny detection rat was somewhat lower in PpGl. Pseudomonas aeruginosa TP can integrate into the PpGl chromosome, producing inducible lysogens. The presence of RP4 is not necessary for the expression of the TP genome in PpGl cells. The D3112cts15 TP may be used for interspecific transduction of plasmids and chromosomal markers.
- Research Organization:
- All-Union Scientific-Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, USSR
- OSTI ID:
- 5652969
- Journal Information:
- Sov. Genet. (Engl. Transl.); (United States), Vol. 21:9; Other Information: Translated from Genetika; 21: No. 9, 1455-1463(Sep 1985)
- Country of Publication:
- United States
- Language:
- English
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550401* - Genetics- Tracer Techniques