Methylation of DNA of maize and wheat grains during fumigation with methyl bromide

Journal Article · · Journal of Agricultural and Food Chemistry; (USA)

DOI:https://doi.org/10.1021/jf00083a032· OSTI ID:7150901

Starratt, A N; Bond, E J ^[1]

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1. Research Centre, London, Ontario (Canada)

The possibility that methylation of DNA occurs during fumigation of foodstuffs with methyl bromide was investigated in two grains, maize and wheat, using {sup 14}C-labeled fumigant. 7-Methylguanine and 1-methyladenine were identified as major products along with lesser amounts of 3-methylcytosine and 3-methyladenine. 3-Methylguanine was probably also formed in minor amounts. Although less than 1% of the bound radioactivity was associated with the DNA isolated, the results indicated that 0.5-1% of the guanine residues in the DNA of these grains was methylated during treatment with 48 mg/L methylbromide for 72 h.

• OSTI ID: 7150901
• Journal Information: Journal of Agricultural and Food Chemistry; (USA), Journal Name: Journal of Agricultural and Food Chemistry; (USA) Vol. 36:5; ISSN JAFCA; ISSN 0021-8561
• Country of Publication: United States
• Language: English

Related Subjects

553004* -- Agriculture & Food Technology-- Food Protection & Preservation-- (1987-)
60 APPLIED LIFE SCIENCES
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CEREALS
CHEMICAL REACTIONS
CYTOSINE
DNA
DRUGS
FUMIGANTS
GRASS
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LILIOPSIDA
MAGNOLIOPHYTA
MAIZE
METHYLATION
NUCLEIC ACIDS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PESTICIDES
PLANTS
PURINES
PYRIMIDINES
REACTION KINETICS
TRACER TECHNIQUES
WHEAT