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Derivatization of vinyl aldehydes with anthrone prior to high-performance liquid chromatography with fluorometric detection

Journal Article · · Anal. Chem.; (United States)
DOI:https://doi.org/10.1021/ac00158a004· OSTI ID:7149255

Precolumn high-performance liquid chromatography derivatization of several vinyl aldehydes, specifically acrolein, crotonaldehyde, and methacrolein, has been studied by use of the reagent anthrone. After selective condensation of these ..cap alpha..- or ..beta..-unsaturated aldehydes to form fluorescent benzanthrone derivatives, separation of these compounds was facile on a 10-cm C-18 column. Although ultraviolet detection was possible, fluorescent detection proved more versatile and sensitive. Four liquor samples were subsequently assayed for acrolein and crotonaldehyde. Linearity for these compounds in alcoholic solutions ranged from 0.02 to at least 14 ppm with detection limits reaching down to 0.005 ppm.

Research Organization:
Miami Univ., Oxford, OH (USA)
OSTI ID:
7149255
Journal Information:
Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 60:7; ISSN ANCHA
Country of Publication:
United States
Language:
English