Effects of estrogen on very low-density lipoprotein triglyceride metabolism in fed and fasted chicks

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:7147091

Park, J R

A single injection of estrogen into growing chicks resulted in a marked elevation in plasma triglyceride (TG) followed by phospholipid (PL) and cholesterol (CH) in both fed and fasted chicks. Estrogen caused a development of massive fatty liver in fed chicks. Hepatic malic enzyme and glucose-6-phosphate dehydrogenase activities also increased significantly in fed chicks and, to a small extent, in fasted chicks. Very low density lipoproteins (VLDL) were barely detectable in the fasted control plasma. However, the VLDL concentration increased markedly upon estrogen injection, becoming the most prevalent lipoprotein in the plasma. The administration of estrogen resulted in an increase in oleic acid and a decrease in linoleic acid content except in the cholesteryl ester of VLDL and LDL. VLDL of estrogenized birds had {beta}-mobility on agarose gel electrophoresis, and they eluted in two peaks on agarose gel filtration chromatography. Both peaks on gel filtration exhibited the same {beta}-mobility on agarose gel electrophoresis. Nevertheless, the apoprotein composition of these two peaks were substantially different from each other; apo B was not present in the first peak VLDL. VLDL-TG kinetic studies conducted in vivo, using {sup 14}C-TG-VLDL prepared endogenously from control and estrogenized chicks revealed that VLDL-TG produced from the former had a higher fractional catabolic rate (FCR) than VLDL-TG from the latter.

• Research Organization: Illinois Univ., Urbana, IL (USA)
• OSTI ID: 7147091
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIRDS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHICKENS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
ESTERS
ESTROGENS
FASTING
FOWL
GEL PERMEATION CHROMATOGRAPHY
GLANDS
HORMONES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LINOLEIC ACID
LIPIDS
LIPOPROTEINS
LIVER
METABOLISM
MONOCARBOXYLIC ACIDS
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PROTEINS
REACTION KINETICS
SEPARATION PROCESSES
STEROID HORMONES
TRACER TECHNIQUES
TRIGLYCERIDES
VERTEBRATES