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Amplification of the structurally and functionally altered epidermal growth factor receptor gene (c-erbB) in human brain tumors

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.8.4.1816· OSTI ID:7143983
By using Southern blot analysis, the authors found that in two cases of human glioblastoma multiforme, cells carried amplified c-erbB genes which bore short deletion mutations within the ligand-binding domain of the epidermal growth factor (EGF) receptor. The products of these mutated c-erB genes were about 30 kilodalton (kDa) smaller than the normal 170-kDa EGF receptor, and the tumor cell membrane fractions containing the 140-kDa abnormal EGF receptor showed a significant elevation of tyrosine kinase activity without its ligand. In view of the similarity to the activated viral and cellular erbB genes in the avian system, these mutated and overexpressed EGF receptors might play a role in the onset or development of human glioblastoma cells.
Research Organization:
Dept. of Genetics, Institute of Medical Science, The Univ. of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108 (JP)
OSTI ID:
7143983
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:4; ISSN MCEBD
Country of Publication:
United States
Language:
English

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