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Tumor promoter and epidermal growth factor stimulate phosphorylation of the c-erbB-2 gene product in MKN-7 human adenocarcinoma cells

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.8.3.1019· OSTI ID:7143942
; ; ; ;
Treatment of human adenocarcinoma MKN-7 cells with epidermal growth factor (EGF) or phorbol tetradecanoate acetate (TPA) stimulated phosphorylation of the c-erbB-2 gene product. EGF induced a rapid increase in phosphotyrosine followed by relatively gradual increases in phosphoserine and phosphothreonine. On the other hand, the TPA-induced increase in phosphorylations occurred exclusively on serine and threonine residues. Tryptic phosphopeptide mapping analysis suggested that treatments with EGF and TPA induced phosphorylation of many common sites in the c-erbB-2 gene product. However, in contrast to TPA, EGF increased the phosphorylation of the c-erbB-2 protein in cells whose protein kinase C had been down-regulated by long-term pretreatment with TPA, suggesting that EGF and TPA induce phosphorylation by different mechanisms. Since the c-erbB-2 gene product did not show detectable EGF-binding activity, phosphorylation of tyrosine of the c-erbB-2 gene product might be catalyzed directly by the EGF receptor kinase that was activated by EGF.
Research Organization:
Institute for Virus Research, Kyoto Univ., Kawaracho, Shogoin, Sakyo-ku, Kyoto 606 (JP)
OSTI ID:
7143942
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:3; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
BIOLOGICAL FUNCTIONS
CARBOXYLIC ACIDS
CARCINOGENS
CARCINOMAS
CHEMICAL REACTIONS
DISEASES
ENZYME ACTIVITY
ENZYMES
ESTERS
FUNCTIONS
GENE AMPLIFICATION
GENE RECOMBINATION PROTEINS
GENES
GROWTH FACTORS
HYDROXY ACIDS
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RECEPTORS
SERINE
STIMULATION
THREONINE
TRANSFERASES
TUMOR CELLS
TYROSINE