Graft-versus-host disease and sialodacryoadenitis viral infection in bone marrow transplanted rats

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-198806000-00003· OSTI ID:7138698

Rossie, K M; Sheridan, J F; Barthold, S W; Tutschka, P J

The effect of a localized viral infection on the occurrence of graft-vs.-host disease (GVHD) was examined in allogeneic rat bone marrow chimeras (ACI/LEW). Animals without clinical evidence of GVHD, 62 days after bone marrow transplant, were infected in salivary and lacrimal glands with sialodacryoadenitis virus (SDAV), and sacrificed 8-25 days postinfection. Using established histologic criteria, GVHD was found more frequently in salivary and lacrimal glands of SDAV-infected chimeras than uninfected chimeras. Skin and oral mucosa, tissues not infected by the virus, showed no differences in occurrence of GVHD, suggesting that the viral infection induced only local and not systemic GVHD. GVHD and SDAV infection, which are histologically similar, were differentiated by examining tissues for SDAV antigen using immunoperoxidase technique. Histologic changes were present for at least 1 week longer than viral antigen, suggesting they represented GVHD rather than viral infection. GVHD and SDAV infection were also differentiated by looking for a histologic feature characteristic of GVHD and not found in SDAV infection (periductal lymphocytic infiltrate). This was found in SDAV-infected chimeras more frequently than uninfected chimeras, suggesting that the viral infection somehow induced GVHD. Results showed a localized increase in the occurrence of GVHD subsequent to localized viral infection.

• Research Organization: Ohio State Univ., Columbus (USA)
• OSTI ID: 7138698
• Journal Information: Transplantation; (United States), Vol. 45:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW
TRANSPLANTS
VIRAL DISEASES
BIOLOGICAL EFFECTS
GRAFT-HOST REACTION
RADIATION CHIMERAS
RATS
SALIVARY GLANDS
ANIMAL TISSUES
ANIMALS
BODY
CHIMERAS
DISEASES
GLANDS
HEMATOPOIETIC SYSTEM
INFECTIOUS DISEASES
MAMMALS
MOSAICISM
ORGANS
RODENTS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals