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Title: Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues

Abstract

Simian acquired immune deficiency syndrome (SAIDS) in rhesus macaques (Macaca mulatta) at the California Primate Research Center is caused by a type D retrovirus designated SAIDS retrovirus serotype 1 (SRV-1). This syndrome is characterized by profound immunosuppression and death associated with opportunistic infections. Neurologic signs and lesions have not been described as part of this syndrome. The distribution of SRV-1 in the salivary glands, lymph nodes, spleens, thymuses, and brains of eight virus-infected rhesus macaques was examined by immunohistochemistry. Electron microscopy, in situ RNA hybridization, and Southern blot hybridization were also performed on selected tissues to detect viral particles, RNA, and DNA, respectively. In seven of eight SRV-1-infected animals, the transmembrane envelope glycoprotein (gp20) of SRV-1 was present in three or more tissues, but never in the brain. In the remaining animal, no viral antigen was detected in any tissue. In this same group of animals, viral nucleic acid was detected in the lymph nodes of six of six animals by Southern blot hybridization, in the salivary glands of two of five animals by both Southern blot and in situ hybridizations, and, surprisingly, in the brains of three of three animals by Southern blot and of three of five animalsmore » by in situ hybridization, including the one animal in which viral gp20 was undetectable. None of these animals had neurologic signs or lesions. The detection of viral nucleic acid in the absence of viral antigen in the brain suggests latent SRV-1 infection of the central nervous system.« less

Authors:
; ; ; ; ; ; ; ; ;  [1]
  1. (Univ. of California, Davis (USA))
Publication Date:
OSTI Identifier:
6522739
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Virology; (USA); Journal Volume: 62:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; AUTORADIOGRAPHY; LYMPH NODES; SALIVARY GLANDS; VIRUSES; TISSUE DISTRIBUTION; CYTOCHEMISTRY; DNA HYBRIDIZATION; MONKEYS; RNA; SULFUR 35; TRANSMISSION ELECTRON MICROSCOPY; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOCHEMISTRY; BODY; CENTRAL NERVOUS SYSTEM; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; ELECTRON MICROSCOPY; EVEN-ODD NUCLEI; GLANDS; HYBRIDIZATION; ISOTOPES; LIGHT NUCLEI; LYMPHATIC SYSTEM; MAMMALS; MICROORGANISMS; MICROSCOPY; NERVOUS SYSTEM; NUCLEI; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PARASITES; PRIMATES; RADIOISOTOPES; SULFUR ISOTOPES; VERTEBRATES; 550701* - Microbiology- Tracer Techniques

Citation Formats

Lackner, A.A., Rodriguez, M.H., Bush, C.E., Munn, R.J., Kwang, Hweising, Moore, P.F., Osborn, K.G., Marx, P.A., Gardner, M.B., and Lowenstine, L.J. Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues. United States: N. p., 1988. Web.
Lackner, A.A., Rodriguez, M.H., Bush, C.E., Munn, R.J., Kwang, Hweising, Moore, P.F., Osborn, K.G., Marx, P.A., Gardner, M.B., & Lowenstine, L.J. Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues. United States.
Lackner, A.A., Rodriguez, M.H., Bush, C.E., Munn, R.J., Kwang, Hweising, Moore, P.F., Osborn, K.G., Marx, P.A., Gardner, M.B., and Lowenstine, L.J. Wed . "Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues". United States. doi:.
@article{osti_6522739,
title = {Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues},
author = {Lackner, A.A. and Rodriguez, M.H. and Bush, C.E. and Munn, R.J. and Kwang, Hweising and Moore, P.F. and Osborn, K.G. and Marx, P.A. and Gardner, M.B. and Lowenstine, L.J.},
abstractNote = {Simian acquired immune deficiency syndrome (SAIDS) in rhesus macaques (Macaca mulatta) at the California Primate Research Center is caused by a type D retrovirus designated SAIDS retrovirus serotype 1 (SRV-1). This syndrome is characterized by profound immunosuppression and death associated with opportunistic infections. Neurologic signs and lesions have not been described as part of this syndrome. The distribution of SRV-1 in the salivary glands, lymph nodes, spleens, thymuses, and brains of eight virus-infected rhesus macaques was examined by immunohistochemistry. Electron microscopy, in situ RNA hybridization, and Southern blot hybridization were also performed on selected tissues to detect viral particles, RNA, and DNA, respectively. In seven of eight SRV-1-infected animals, the transmembrane envelope glycoprotein (gp20) of SRV-1 was present in three or more tissues, but never in the brain. In the remaining animal, no viral antigen was detected in any tissue. In this same group of animals, viral nucleic acid was detected in the lymph nodes of six of six animals by Southern blot hybridization, in the salivary glands of two of five animals by both Southern blot and in situ hybridizations, and, surprisingly, in the brains of three of three animals by Southern blot and of three of five animals by in situ hybridization, including the one animal in which viral gp20 was undetectable. None of these animals had neurologic signs or lesions. The detection of viral nucleic acid in the absence of viral antigen in the brain suggests latent SRV-1 infection of the central nervous system.},
doi = {},
journal = {Journal of Virology; (USA)},
number = ,
volume = 62:6,
place = {United States},
year = {Wed Jun 01 00:00:00 EDT 1988},
month = {Wed Jun 01 00:00:00 EDT 1988}
}