v-src induces clonal sarcomas and rapid metastasis following transduction with a replication-defective retrovirus
- Univ. of California, Berkeley (USA)
v-src is an effective carcinogen when expressed from Rous sarcoma virus (RSV) in vivo. Whereas RSV tumors require sustained oncogene expression, their growth is largely a balance between viral recruitment of tissues and host immune destruction of infected cells. The authors have therefore examined the tumorigenic potential of v-src in the absence of viral recruitment and viral antigen expression. v-src was introduced with high efficiency into chicken wing web tissues using replication-defective (rd) retroviral vectors. Clonal sarcomas were induced rapidly, and furthermore, v-src potentiated metastatic progression in {approx} 0.1%-1% of tumor clones with unexpectedly short latency. rd vectors proved effective not only in transducing v-src into tissues but also as insertional markers of tumor clonality. The rd vector present in most primary and metastatic tumors was a highly truncated form of RSV derived by viral transmission of spliced v-src mRNA; this vector should thus avoid viral recruitment and host anti-viral immune reaction through its complete lack of viral structural genes. Under such conditions v-src maintains strong carcinogenicity in vivo when restricted to clonal tumor growth and can confer rapid metastatic potential on a discrete subset of tumor clones.
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 7138300
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:24; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
Similar Records
Rous sarcoma virus transforming protein tyrosine kinase is expressed and active in sarcoma-free avian embryos microinjected with Rous sarcoma virus
Sequence comparison in the crossover region of an oncogenic avian retrovirus recombinant and its nononcogenic parent: Genetic regions that control growth rate and oncogenic potential
Related Subjects
CARCINOGENESIS
ETIOLOGY
DNA
AUTORADIOGRAPHY
ONCOGENES
GENE AMPLIFICATION
ANTIGENS
DNA POLYMERASES
METASTASES
ONCOGENIC VIRUSES
PHOSPHORUS 32
SARCOMAS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
GENES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PATHOGENESIS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
TRANSFERASES
VIRUSES
550200* - Biochemistry