(Molecular characteristics of the lignin forming peroxidase)
Since this manuscript was submitted we have conducted a more thorough physiological analysis of water relations in wild-type and peroxidase overproducing plants. These experiments include pressure bomb, plasmolysis, and membrane integrity analysis. We are also in the process of analyzing other phenotypes in peroxidase overproducer plants such as excessive browning of tissue, the rapid death of tissue in culture, and poor germination of seed. Transformed plants of Nicotiana tabacum and Nicotiana sylvestris were obtained which have peroxidase activity 3--7 fold lower than wild-type plants. This was done by introducing a chimeric gene composed of the CaMV 35S promoter and the 5' half of the tobacco anionic peroxidase cDNA in the antisense RNA configuration. A manuscript which describes this work is being written, and will be submitted for publication in January 1990. The anionic peroxidase gene has been cloned by hybridization to the cloned cDNA. The entire gene is contained on an 8.7kb fragment within a lambda phage clone. Several smaller DNA fragments have been subcloned, and some have been sequenced. One exon within the coding sequence has been sequenced, along with the partial sequence of two introns. Further sequencing is being carried-out to identify the promoter, which will be later joined to a reporter gene. 6 figs.
- Research Organization:
- Ohio State Univ. Research Foundation, Columbus, OH (USA)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG02-89ER14004
- OSTI ID:
- 7138283
- Report Number(s):
- DOE/ER/14004-1; ON: DE90010880
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
CELL TRANSFORMATIONS
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
ENZYME INHIBITORS
ENZYMES
GENETIC MAPPING
HYBRIDIZATION
INHIBITION
LIGNIN
MAPPING
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
POLYSACCHARIDES
PROGRESS REPORT
RNA
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
TOBACCO