Indium-111-labeled LDL: A potential agent for imaging atherosclerotic disease and lipoprotein biodistribution

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Journal of Nuclear Medicine; (USA)

OSTI ID:7137583

Rosen, J M; Butler, S P; Meinken, G E; Wang, T S; Ramakrishnan, R; Srivastava, S C; Alderson, P O; Ginsberg, H N ^[1]

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1. Columbia Univ. College of Physicians and Surgeons, New York, NY (USA)

Radiolabeling of low-density lipoprotein (LDL) and external imaging with a gamma camera would offer a means of taking advantage of the metabolic activity of developing atherosclerotic lesions in order to noninvasively detect and determine the extent of atherosclerotic cardiovascular disease. Indium-111-({sup 111}In) labeled LDL was prepared and its purity demonstrated by agarose electrophoresis and ultracentrifugation. In vitro studies with cultured human fibroblasts demonstrated significant inhibition of iodine-125-({sup 125}I) LDL binding to LDL receptors by {sup 111}In-LDL, although this was less than the inhibition produced by unlabeled LDL. Adrenal gland uptake of {sup 111}In-LDL by hypercholesterolemic rabbits was reduced by 86% compared to the level of uptake observed in normal rabbits. These results were compatible with downregulation of adrenal LDL receptors in the hypercholesterolemic rabbits. Uptake of {sup 111}In-LDL in the atherosclerotic proximal aorta of hypercholesterolemic rabbits was 2.5 times higher than in normal rabbits. These results suggest that {sup 111}In-LDL has the potential to be a useful agent for external imaging of atherosclerotic lesions and lipoprotein biodistribution.

• OSTI ID: 7137583
• Journal Information: Journal of Nuclear Medicine; (USA), Journal Name: Journal of Nuclear Medicine; (USA) Vol. 31:3; ISSN 0161-5505; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL GLANDS
AMINO ACIDS
ANIMALS
ARTERIOSCLEROSIS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CENTRIFUGATION
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
DISTRIBUTION
DRUGS
DTPA
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENDOCRINE GLANDS
GLANDS
IMAGE PROCESSING
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KINETICS
LIPIDS
LIPOPROTEINS
MAMMALS
MEMBRANE PROTEINS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROCESSING
PROTEINS
RABBITS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RECEPTORS
SEPARATION PROCESSES
TISSUE DISTRIBUTION
ULTRACENTRIFUGATION
VASCULAR DISEASES
VERTEBRATES