Radiotracers for low density lipoprotein biodistribution studies in vivo: technetium-99m low density lipoprotein versus radioiodinated low density lipoprotein preparations

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · J. Nucl. Med.; (United States)

OSTI ID:6902505

Vallabhajosula, S; Paidi, M; Badimon, J J; Le, N A; Goldsmith, S J; Fuster, V; Ginsberg, H N

In an attempt to characterize the in vivo behavior of (99mTc) low density lipoprotein (LDL), biodistribution studies were performed in normal and hypercholesterolemic (HC) rabbits. In normal rabbits, 24 hr after the injection of (99mTc)LDL, 99mTc activity accumulated mainly in adrenal glands, spleen, liver, and kidney. In HC rabbits, however, there was a marked reduction of 99mTc activity in these organs. In both normal and HC rabbits, less than 17% of 99mTc activity appeared in the 24-hr urine following injection of (99mTc)LDL, suggesting that in vivo, (99mTc)LDL is trapped and accumulated within the tissues. Direct comparison of (99mTc)LDL, 125I-native-LDL and (131I)tyramine cellobiose-LDL (the previously validated trapped radioligand) in normal rabbits, demonstrated that the biodistribution of (99mTc)LDL was similar to that of (131I)tyramine cellobiose-LDL. The adrenal glands, liver, and spleen accumulated significantly greater quantities of 99mTc and 131I activity per gram of tissue than 125I (from native-LDL). In addition, imaging studies in monkeys, showed that the hepatic uptake and retention of (99mTc) LDL was similar to that of (131I)tyramine cellobiose LDL. In contrast, radioiodine from native-LDL was deiodinated in liver with subsequent excretion into the intestine. These results suggest that (99mTc)LDL acts as a trapped ligand in vivo and should therefore, be a good tracer for noninvasive quantitative biodistribution studies of LDL.

• Research Organization: Mount Sinai School of Medicine, New York, NY (USA)
• OSTI ID: 6902505
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 29:7; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELLOBIOSE
CHOLESTEROL
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DISACCHARIDES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HOURS LIVING RADIOISOTOPES
HYDROXY COMPOUNDS
IN VIVO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
LIPOPROTEINS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PHENOLS
PROTEINS
RABBITS
RADIOISOTOPES
SACCHARIDES
STEROIDS
STEROLS
SYMPATHOMIMETICS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TYRAMINE
VERTEBRATES
YEARS LIVING RADIOISOTOPES