Kupffer cell proliferation and glucan-induced granuloma formation in mice depleted of blood monocytes by strontium-89
- Kumamoto Univ. Medical School (Japan)
In mice with prolonged severe monocytopenia induced by selective irradiation of the bone marrow with the bone-seeking isotope 89Sr, the proliferative capacity of Kupffer cells was studied by immunohistochemistry with an anti-mouse macrophage monoclonal antibody, F4/80, ultrastructural peroxidase (PO) cytochemistry, and tritiated thymidine (3HTdR) autoradiography. The number and 3HTdR uptake of Kupffer cells were significantly increased in the splenectomized mice after severe monocytopenia had continued for more than 4 wk, and almost all the Kupffer cells showed a localization pattern of PO activity similar to that of resident macrophages in the liver of normal mice. In the glucan-induced granuloma formation in similar monocytopenic mice, Kupffer cells proliferated, conglomerated, and transformed into epithelioid cells, which fused together to become multinuclear giant cells. These results suggest that Kupffer cells are a self-renewing population by their own cell division and can participate actively in granulomatous inflammations in severely monocytopenic and intact mice.
- OSTI ID:
- 7136243
- Journal Information:
- Journal of Leukocyte Biology; (USA), Journal Name: Journal of Leukocyte Biology; (USA) Vol. 47:3; ISSN 0741-5400; ISSN JLBIE
- Country of Publication:
- United States
- Language:
- English
Similar Records
Resident macrophage proliferation in mice depleted of blood monocytes by strontium-89
Restoration of prostaglandin releasing macrophage populations in lethally irradiated mice with spleen cells from bone marrow-depleted donors
Related Subjects
560162* -- Radionuclide Effects
Kinetics
& Toxicology-- Animals
Plants
Microorganisms
& Cells
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
EVEN-ODD NUCLEI
GRANULOMAS
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MICE
MONOCLONAL ANTIBODIES
MONOCYTES
NEOPLASMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PHAGOCYTES
PYRIMIDINES
RADIOINDUCTION
RADIOISOTOPES
RIBOSIDES
RODENTS
SOMATIC CELLS
STEM CELLS
STRONTIUM 89
STRONTIUM ISOTOPES
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES