Resident macrophage proliferation in mice depleted of blood monocytes by strontium-89
The capacity of resident peritoneal macrophages and alveolar macrophages for self-renewal was studied in CD-1 mice depleted of radiosensitive blood monocyte precursors by irradiation of the bone marrow with the bone-seeking isotope 89Sr. Thermoluminescent dosimetry and studies of DNA synthesis showed that significant levels of radiation were absorbed predominantly in anatomical sites in close proximity to bone. Cell proliferation remote from bone marrow was unaffected when quantitated by DNA precursor uptake. Between 10 and 15 days after injecting mice intravenously with 89Sr at 2 microCi per gm. of body weight, monocytes were only rarely detectable in the peripheral blood as determined by morphology, stains for nonspecific esterase and peroxidase, and by latex phagocytosis. Total numbers of resident peritoneal and alveolar macrophages were not significantly decreased and tritiated thymidine incorporation in vivo persisted at normal levels rather than regressing at a predicted rate during profound monocyte depletion. The data, therefore, suggest that local proliferation by resident macrophages in monocytopenic and intact mice is an important mechanism of population renewal.
- Research Organization:
- East Carolina University School of Medicine, Greenville, North Carolina
- OSTI ID:
- 5294795
- Journal Information:
- Lab. Invest.; (United States), Journal Name: Lab. Invest.; (United States) Vol. 46:2; ISSN LAINA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DOSIMETRY
EVEN-ODD NUCLEI
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
LEUKOPENIA
MACROPHAGES
MAMMALS
MATERIALS
MICE
MONOCYTES
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
RADIATION EFFECTS
RADIOINDUCTION
RADIOISOTOPES
RODENTS
SOMATIC CELLS
STRONTIUM 89
STRONTIUM ISOTOPES
SYNTHESIS
THERMOLUMINESCENT DOSIMETRY
VERTEBRATES