Enhanced expression of c-myc and decreased expression of c-fos protooncogenes in chemically and radiation-transformed C/sub 3/H/10T1/2 Cl 8 mouse embryo cell lines

Journal Article · Wed Oct 01 04:00:00 EDT 1986 · Cancer Res.; (United States)

OSTI ID:7133110

Shuin, T; Billings, P C; Lillehaug, J R; Patierno, S R; Roy-Burman, P; Landolph, J R

c-abl, c-fos, c-Ha-ras, c-myc, and c-mos were expressed whereas c-sis, c-fms, c-rel, c-src, and c-myb expression was not detectable in C/sub 3/H/10T1/2 Cl 8 (10T1/2) cells and in eight chemically and radiation-transformed 10T1/2 cell lines. The expression of c-abl, c-fos, c-Ha-ras, and c-myc was growth-related in nontransformed 10T1/2 cells. c-abl and c-fos expression increased at confluence by 5- and 9-fold, respectively, compared to that in log phase cells. c-Ha-ras and c-myc transcripts were most abundant in log phase cells and decreased by 70 and 50%, respectively, in confluent cells. There were no significant growth-related changes in the expression of c-Ha-ras, c-myc, or c-abl in methylcholanthrene-transformed Cl 15 cells. The c-fos transcript was not detected in Cl 15 cell cultures. c-abl, c-fos, c-ras, and c-myc were expressed in whole C3H mouse embryo tissue, mouse liver, and 10T1/2 cells. Sizes of these protooncogene transcripts in 10T1/2 cells were the same as those in whole embryo tissue, except that 10T1/2 cells did not express the 8.2-kilobase abl transcript. At subconfluence, equivalent low levels of c-mos expression were observed in nontransformed and in the eight transformed 10T1/2 cell lines. The level of c-abl expression was similar in the nontransformed and in the eight transformed cell lines, but there was a new 8.2-kilobase transcript in the transformed MCA Cl 15 cell line. c-fos was expressed in 10T1/2 cells but was not detectable or greatly reduced in eight transformed cell lines. c-Ha-ras was expressed to a similar extent in eight transformed cell lines and in nontransformed 10T1/2 cells. In the UVC-4 transformed cell line, extra 3.3-kilobase Ha-ras and 7.5-kilobase Ki-ras transcripts were observed. c-myc was expressed at 4- to 7-fold higher levels in six transformed cell lines compared to 10T1/2 cells.

• Research Organization: Univ. of Southern California, Los Angeles
• OSTI ID: 7133110
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 10; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GROWTH
HYDROCARBONS
MAMMALS
MICE
ONCOGENES
ORGANIC COMPOUNDS
PHENOTYPE
POLYCYCLIC AROMATIC HYDROCARBONS
RADIATION EFFECTS
RODENTS
VERTEBRATES