Collagen binding to Staphylococcus aureus
Staphylococcus aureus can bind soluble collagen in a specific, saturable manner. We have previously shown that some variability exists in the degree of collagen binding between different strains of heat-killed, formaldehyde-fixed S. aureus which are commercially available as immunologic reagents. The present study demonstrates that live S. aureus of the Cowan 1 strain binds amounts of collagen per organism equivalent to those demonstrated previously in heat-killed, formaldehyde-fixed bacteria but has an affinity over 100 times greater, with Kd values of 9.7 X 10(-11) M and 4.3 X 10(-8) M for live and heat-killed organisms, respectively. Studies were also carried out with S. aureus killed by ionizing radiation, since this method of killing the organism seemed less likely to alter the binding moieties on the surface than did heat killing. Bacteria killed by exposure to gamma radiation bound collagen in a manner essentially indistinguishable from that of live organisms. Binding of collagen to irradiated cells of the Cowan 1 strain was rapid, with equilibrium reached by 30 min at 22 degrees C, and was fully reversible. The binding was not inhibited by fibronectin, fibrinogen, C1q, or immunoglobulin G, suggesting a binding site for collagen distinct from those for these proteins. Collagen binding was virtually eliminated in trypsin-treated organisms, indicating that the binding site has a protein component. Of four strains examined, Cowan 1 and S. aureus ATCC 25923 showed saturable, specific binding, while strains Woods and S4 showed a complete lack of binding. These results suggest that some strains of S. aureus contain high-affinity binding sites for collagen. While the number of binding sites per bacterium varied sixfold in the two collagen-binding strains, the apparent affinity was similar.
- Research Organization:
- Cleveland Clinic Foundation, OH
- OSTI ID:
- 7132994
- Journal Information:
- Infect. Immun.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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COLLAGEN
CHEMICAL BONDS
STAPHYLOCOCCUS
BIOLOGICAL RADIATION EFFECTS
CELL KILLING
FORMALDEHYDE
GAMMA RADIATION
RECEPTORS
TEMPERATURE EFFECTS
ALDEHYDES
BACTERIA
BIOLOGICAL EFFECTS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MEMBRANE PROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
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RADIATION EFFECTS
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560130* - Radiation Effects on Microorganisms
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