Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.
Balachandran, Manasi, et al. "Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46." PLoS ONE, vol. 12, no. 8, Aug. 2017. https://doi.org/10.1371/journal.pone.0183913
Balachandran, Manasi, Giannone, Richard J., Bemis, David A., & Kania, Stephen A. (2017). Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46. PLoS ONE, 12(8). https://doi.org/10.1371/journal.pone.0183913
Balachandran, Manasi, Giannone, Richard J., Bemis, David A., et al., "Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46," PLoS ONE 12, no. 8 (2017), https://doi.org/10.1371/journal.pone.0183913
@article{osti_1394588,
author = {Balachandran, Manasi and Giannone, Richard J. and Bemis, David A. and Kania, Stephen A.},
title = {Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46},
annote = {Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.},
doi = {10.1371/journal.pone.0183913},
url = {https://www.osti.gov/biblio/1394588},
journal = {PLoS ONE},
issn = {ISSN 1932-6203},
number = {8},
volume = {12},
place = {United States},
publisher = {Public Library of Science},
year = {2017},
month = {08}}
Amend, Alfred; Chhatwal, Gursharan S.; Schaeg, Werner
Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology, Vol. 258, Issue 4https://doi.org/10.1016/S0176-6724(84)80024-3