Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46

Balachandran, Manasi; Giannone, Richard J.; Bemis, David A.; Kania, Stephen A.

doi:10.1371/journal.pone.0183913

Title: Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46

Journal Article · Thu Aug 31 00:00:00 EDT 2017 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0183913· OSTI ID:1394588

Balachandran, Manasi ^[1]; Giannone, Richard J. ^[2]; Bemis, David A. ^[1];

^[1]

Univ. of Tennessee, Knoxville, TN (United States). Dept. of Biomedical and Diagnostic Sciences
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division. Mass Spectrometry and Laser Spectrometry

Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.

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Research Organization:: Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; Tennessee Center of Excellence in Livestock Diseases and Human Health (United States)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1394588

Journal Information:: PLoS ONE, Vol. 12, Issue 8; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 4 works

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Title: Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46

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