Long-term physiological effects of enhanced O/sub 2/ release by inositol hexaphosphate-loaded erythrocytes
A continuous lysing and resealing procedure with erythrocytes permitted incorporation in these cells of inositol hexaphosphate (InsP/sub 6/), a strong allosteric effector of Hb. This leads to significant rightward shifts of the HbO/sub 2/ dissociation curves with in vitro P/sub 50/, values increasing from 32.2 +/- 1.8 torr for control erythrocytes to 86 +/- 60 torr. The shape of the dissociation curve was still sigmoidal, although the Hill coefficient was decreased. The life span of InsP/sub 6/-loaded erythrocytes equaled that of control erythrocytes. Erythrocyte-survival studies were done using /sub 51/Cr labeling of cells. The long-term physiological effects of the InsP/sub 6/-loaded erythrocytes on piglets were increased O/sub 2/ release and reduced cardiac output. The reduced O/sub 2/ affinity of the InsP/sub 6/-loaded erythrocytes was still effective 20 days after transfusion in awake piglets. The electrolyte concentration appeared stable over the 5-day observation period except for a transient, but significant, hyperkalemia immediately after transfusion. The reductions in the O/sub 2/ affinity of Hb reported here are large compared with previously reported values. Introduction of InsP/sub 6/ into viable erythrocytes improves tissue oxygenation when, for any reason, normal blood flow is impaired.
- Research Organization:
- Hospital Henri Mondor, Creteil (France)
- OSTI ID:
- 7130985
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:19
- Country of Publication:
- United States
- Language:
- English
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ERYTHROCYTES
LABELLING
PHYSIOLOGY
OXYGEN
BIOLOGICAL EFFECTS
CHROMIUM 51
HEMOGLOBIN
INOSITOL
MAN
SWINE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
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CHROMIUM ISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INOSITOLS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MATERIALS
MONOSACCHARIDES
NONMETALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PRIMATES
PROTEINS
RADIOISOTOPES
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551001* - Physiological Systems- Tracer Techniques