Study of abnormal plasma low-density lipoprotein in rhesus monkeys with diet-induced hyperlipidemia
Male rhesus monkeys were divided into three groups: five were fed a regular primate chow diet and were used as controls; four received an ''average'' American diet; and five a special low-fat primate chow diet supplemented with 25 percent coconut oil and 2 percent cholesterol. In all of these animals, the plasma low-density lipoproteins (LDL) were isolated by ultracentrifugal flotation between densities of 1.019 and 1.050 g/ml. The LDL of the five control monkeys had variable molecular weights, with a mean value of 3.12 +- 0.21 x 10/sup 6/ (range: 2.92 x 10/sup 6/ to 3.45 x 10/sup 6/), and an average partial specific volume of 0.969 +- 0.003 ml/g; both were assessed by flotation equilibrium analysis in the analytical ultracentrifuge. In the individual animals, however, the physical properties of LDL were invariant with time. The administration of either an ''average'' American diet or a coconut oil-cholesterol diet was accompanied by hypercholesterolemia associated with changes in LDL which were characterized by increases in molecular weight to 3.52 +- 0.21 x 10/sup 6/ (average of nine monkeys) and in partial specific volume to 0.973 +- 0.002 ml/g.
- Research Organization:
- Univ. of Chicago
- OSTI ID:
- 7126196
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 15:26; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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