Measurement of rhesus monkey (Macaca mulatta) apolipoprotein B in serum by radioimmunoassay: comparison of immunoreactivities of rhesus and human low density lipoproteins

Journal Article · Wed Feb 01 04:00:00 EST 1978 · J. Lipid Res.; (United States)

OSTI ID:5761349

Karlin, J B; Juhn, D J; Fless, G; Scanu, A M; Rubenstein, A H

A sensitive and specific double antibody radioimmunoassay for the major apolipoprotein (apoB) of rhesus (Macaca mulatta) serum very low density lipoprotein (VLDL) and low density lipoprotein (LDL) is described. The antiserum was raised to LDL (d 1.030 to 1.040 g/ml) and the LDL/sub 2/ (d 1.020 to 1.050 g/ml) was labeled with /sup 125/I by the chloramine-T or iodine monochloride method. The assay, which was sensitive to 0.02 to 0.5 ..mu..g of LDL/sub 2/, had an interassay coefficient of variation of 4.5%. This assay was successfully used to measure apoB in the whole serum and low density lipoproteins of control monkeys maintained on a standard Purina monkey chow (PMC) diet and of three groups of monkeys fed atherogenic diets: an average American diet, a 25% peanut oil and 2% cholesterol-supplemented PMC diet, and a 25% coconut oil and 2% cholesterol-supplemented PMC diet.

• Research Organization: Univ. of Chicago, IL
• OSTI ID: 5761349
• Journal Information: J. Lipid Res.; (United States), Journal Name: J. Lipid Res.; (United States) Vol. 19:2; ISSN JLPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTERIOSCLEROSIS
CARDIOVASCULAR DISEASES
CHOLESTEROL
DISEASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LIPIDS
LIPOPROTEINS
MAMMALS
MONKEYS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
STEROIDS
STEROLS
TRACER TECHNIQUES
VERTEBRATES