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U.S. Department of Energy
Office of Scientific and Technical Information

Inactivation of enteric viruses in wastewater sludge

Conference ·
OSTI ID:7124642
The purpose of the work being carried out at Sandia Laboratories on enteric viruses is to find the most plausible methods of inactivating these pathogens in sludge. This study is feasible only when using sludge seeded with a large number of infectious viruses and is meaningful only when virus recovery is monitored using purified, radioactively labeled viruses. Using these techniques it is possible to determine both the rate and mechanism of virus inactivation expected during each stage of sludge treatment. The results presented here have been obtained during the initial phase of this work. It has been shown that both raw and digested sludge contain an agent with cidal activity against enteroviruses whose expression is dependent on pH. This agent is the uncharged form of ammonia. The presence of this agent greatly accelerates the rate of heat inactivation of viruses in sludge. Because anaerobic digestion of sludge typically occurs at pH 7, a pH at which ammonia is in its charged, non-virucidal state, this agent probably contributes little toward the inactivation of viruses during the anaerobic digestion process.
Research Organization:
Sandia Labs., Albuquerque, NM (USA)
DOE Contract Number:
E(29-1)-789
OSTI ID:
7124642
Report Number(s):
SAND-76-5748; CONF-761211-1
Country of Publication:
United States
Language:
English