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In vitro assessment of zinc binding to protein foods as a potential index of zinc bioavailability. Comparison of in vitro and in vivo data

Journal Article · · J. Agric. Food Chem.; (United States)
DOI:https://doi.org/10.1021/jf00066a026· OSTI ID:7119038
; ;

An in vitro equilibrium dialysis test for estimating the strength of zinc binding to protein foods was developed for predicting zinc bioavailability. Soy flour, soy concentrate, casein, and dried egg white were labeled with /sup 65/ZnCl/sub 2/ before dialysis. The conditions included 24-h dialysis at pH 7.4 against 0.05 M tris(hydroxymethyl)aminomethane buffer (Tris), Tris plus 0.01 M L-histidine hydrochloride (Tris-His), and Tris plus 0.01 M Na/sub 2/EDTA (Tris-EDTA). Dialyzate and retentate /sup 65/Zn were measured. The protein foods retained /sup 65/Zn in the following decreasing order according to treatment: Tris > Tris-His > Tris-EDTA. The bioavailability of residual /sup 65/Zn in casein, egg white, soy concentrate, and soy flour after each buffer treatment was determined by giving single doses of the protein foods to young Japanese quail. For these protein foods, the best agreement between in vitro and in vivo data was with Tris-His-dialyzable /sup 65/Zn values and the whole-body /sup 65/Zn retentions from the labeled casein and egg white (no treatment). The data suggest that this in vitro test could be useful for preliminary assessment of zinc bioavailability of protein foods.

Research Organization:
Food and Drug Administration, Washington, DC
OSTI ID:
7119038
Journal Information:
J. Agric. Food Chem.; (United States), Journal Name: J. Agric. Food Chem.; (United States) Vol. 33:6; ISSN JAFCA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL AVAILABILITY
BIRDS
CASEIN
CHLORIDES
CHLORINE COMPOUNDS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIALYSIS
EGGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FOOD
HALIDES
HALOGEN COMPOUNDS
IN VITRO
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
METALS
NUCLEI
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PLANTS
PROTEINS
RADIOISOTOPES
RETENTION
SEPARATION PROCESSES
SOYBEANS
TRACER TECHNIQUES
VEGETABLES
VERTEBRATES
ZINC
ZINC 65
ZINC CHLORIDES
ZINC COMPOUNDS
ZINC HALIDES
ZINC ISOTOPES