Extraction, separation, and detections of /sup 14/C-diazepam and /sup 14/C-metabolites from brain tissue of mature and old rats

Journal Article · · J. Anal. Toxicol.; (United States)

OSTI ID:7118895

Komiskey, H L; Rahman, A; Weisenburger, W P; Hayton, W L; Zobrist, R H; Silvius, W

A rapid method for simultaneous determination of brain concentrations of diazepan and each of its three major metabolites in brain tissue by a reverse isotope dilution procedure is presented. Radiolabeled diazepam and metabolites were extracted from brain tissue of mature and senescent rats with ethyl ether. After the ether was evaporated the benzodiazepines were separated from the residue by passing the water soluble portion through C-18 bonded-phase extraction columns. High pressure liquid chromatography (HPLC) was used to separate the benzodiazepines from each other. Reverse isotope dilution analysis was used to quantify diazepam and its metabolites. The percent recovery of diazepam and its metabolites from the brain of mature or senescent rats did not vary significantly.

• Research Organization: Univ. of Illinois, Rockford
• OSTI ID: 7118895
• Journal Information: J. Anal. Toxicol.; (United States), Journal Name: J. Anal. Toxicol.; (United States) Vol. 9:3; ISSN JATOD
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BODY
BRAIN
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COUNTING TECHNIQUES
ELECTROMAGNETIC RADIATION
EXTREME ULTRAVIOLET RADIATION
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLITES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIATIONS
RATS
RODENTS
SCINTILLATION COUNTING
SEPARATION PROCESSES
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
VERTEBRATES