Reverse isotope dilution method for determining benzene and metabolites in tissues

Journal Article · Fri Jul 01 00:00:00 EDT 1988 · J. Anal. Toxicol.; (United States)

DOI:https://doi.org/10.1093/jat/12.4.176· OSTI ID:6597104

Bechtold, W E; Sabourin, P J; Henderson, R F

A method utilizing reverse isotope dilution for the analysis of benzene and its organic soluble metabolites in tissues of rats and mice is presented. Tissues from rats and mice that had been exposed to radiolabeled benzene were extracted with ethyl acetate containing known, excess quantities of unlabeled benzene and metabolites. Butylated hydroxytoluene was added as an antioxidant. The ethyl acetate extracts were analyzed with semipreparative reversed-phase HPLC. Isolated peaks were collected and analyzed for radioactivity (by liquid scintillation spectrometry) and for mass (by UV absorption). The total amount of each compound present was calculated from the mass dilution of the radiolabeled isotope. This method has the advantages of high sensitivity, because of the high specific activity of benzene, and relative stability of the analyses, because of the addition of large amounts of unlabeled carrier analogue.

• Research Organization: Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (USA)
• OSTI ID: 6597104
• Journal Information: J. Anal. Toxicol.; (United States), Journal Name: J. Anal. Toxicol.; (United States) Vol. 12:4; ISSN JATOD
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
AROMATICS
BENZENE
BODY
CHROMATOGRAPHY
DIGESTIVE SYSTEM
GLANDS
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAMMALS
MEASURING METHODS
METABOLISM
MICE
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
SEPARATION PROCESSES
TISSUES
TRACER TECHNIQUES
VERTEBRATES