Tissue distribution and half-life of sup 125 I-endothelin in the rat: Importance of pulmonary clearance

Journal Article · Thu Mar 29 23:00:00 EST 1990 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/0006-291X(90)90649-8· OSTI ID:7118015

Sirvioe, M L.M.; Metsaerinne, K S; Saijonmaa, O; Fyhrquist, F ^[1]

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1. Minerva Foundation Institute for Medical Research, Helsinki (Finland)

Plasma half-life and organ uptake of endothelin 1 (ET-1) was studied in anaesthetized Wistar rats administering a bolus of {sup 125}I-labeled ET-1 into jugular vein, with and without a preceding dose of 1-80 ng of unlabeled ET-1, via the same route. Plasma half-life of intact {sup 125}I-ET-1 was 40 sec and that of total radioactivity 68 sec. Immunoreactivity of circulating {sup 125}I-ET was rapidly reduced, by 23% at 30 sec and by 43% at 360 sec, respectively. High power liquid chromatography of plasma extracts revealed a corresponding decrease of intact {sup 125}I-ET-1 peak with time. There was a predominant uptake of {sup 125}I-ET-1 in the lungs, 82%, and lesser by kidneys, 10%, heart 3.6%, liver, 2.7%, and spleen, 1.5%, respectively. Pretreatment with unlabeled ET-1 caused a dose-dependent shift of uptake from lungs to kidneys. We conclude that circulating ET-1 is rapidly cleared, primarily by the lungs, which may play an important role in protecting the systemic circulation from the vasoconstricting actions of ET-1.

• OSTI ID: 7118015
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 167:3; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL HALF-LIFE
BODY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
TISSUE DISTRIBUTION
TISSUES
VERTEBRATES