Uptake and metabolism of endothelin in the isolated perfused rat lung

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Experimental Lung Research; (USA)

DOI:https://doi.org/10.3109/01902149009068824· OSTI ID:6071827

Westcott, J Y; Henson, J; McMurtry, I F; O'Brien, R F ^[1]

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1. Univ. of Colorado Health Sciences Center, Denver (USA)

The uptake and metabolism of endothelin was studied in the isolated perfused rat lung. {sup 125}I-Labeled endothelin (4 x 10(-12) M) was rapidly removed from the lung perfusate. More than 90% of the label was removed by 5 min, and only 4-5% remained in the perfusate after 30 min. In the presence of 10(-9) or 5 x 10(-9) M unlabeled endothelin added 6 min before {sup 125}I-labeled endothelin, the radiolabel was more slowly removed, so that after 30 min 19 or 73% of the label, respectively, remained in the perfusate. Although a high level of unlabeled endothelin in the perfusate reduced the uptake of {sup 125}I-labeled endothelin, it did not displace radiolabel previously taken up by the lung. Analysis of radiolabel composition showed that endothelin metabolism was less than 15% in both the lung and the perfusate after 30 min of lung perfusion. Autoradiography of the lung indicated that the radiolabel was located primarily within the alveolar wall. These results suggest that circulating endothelin is readily but finitely taken up in the pulmonary microvasculature where it is avidly bound but slowly metabolized.

• OSTI ID: 6071827
• Journal Information: Experimental Lung Research; (USA), Journal Name: Experimental Lung Research; (USA) Vol. 16:5; ISSN EXLRD; ISSN 0190-2148
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LUNGS
MAMMALS
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PERFUSED ORGANS
PROTEINS
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RODENTS
UPTAKE
VERTEBRATES