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Metabolism of arachidonic acid in phorbol ester, interferon and dimethyl sulfoxide differentiation induced U937 cells

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7117942
U937, a human macrophage cell line can metabolize arachidonic acid to a prostaglandin E2-like substance, and an unidentified lipoxygenase product. This metabolism occurs at very low levels however since these cells have low lipase and fatty acid oxygenase activities. The investigated the appearance of these enzyme activities during differentiation induced by phorbol-12-myristate-13-acetate (PMA), human gamma interferon (INF), and dimethyl sulfoxide (DMSO) on days 1,3 and 5 of stimulation using /sup 3/H-arachidonic acid (/sup 3/H-AA). Culture supernatants were analyzed for free /sup 3/H-AA and /sup 3/H metabolites by radio-thin layer chromatography (/sup 3/H-MET). The increasing percentage of /sup 3/H-AA release suggests the appearance of phospholipase activity during differentiation.
Research Organization:
Univ. of Illinois, Chicago
OSTI ID:
7117942
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
Country of Publication:
United States
Language:
English

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