Arachidonic acid is involved in the regulation of hCG induced steroidogenesis in rat Leydig cells

Journal Article · Mon Jul 27 00:00:00 EDT 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90223-2· OSTI ID:6150566

Didolkar, A K; Sundaram, K

Phospholipase C (PLC), an enzyme involved in the hydrolysis of membrane phospholipid- phosphatidylinositol-bisphosphate to insositol triphosphate and diacylglycerol, and Phorbol 12, myristate 13, acetate (PMA) could significantly stimulate testosterone (T) secretion from Leydig cells. Arachidonic acid (AA) stimulated T secretion by about 2 fold. The steroidogenic effect of PLC and AA was biphasic. At low concentrations both PLC and AA augmented hCG induced T secretion, while at higher concentrations they inhibited steroid production. AA also had a biphasic effect on hCG induced cyclic AMP secretion. 5,8,11,14 Eicosatetrayenoic acid, a general inhibitor of AA metabolism, and Nordihydroguaiaretic acid, an inhibitor of the lipoxygenase pathway of AA metabolism, inhibited hCG induced T secretion while indomethacin, an inhibitor of cyclo-oxygenase pathway, had no effect on hCG induced T secretion. The authors conclude from these data that AA plays a role in the regulation of hCG induced steroidogenic responses in rat Leydig cells and that the metabolite(s) of AA that are involved are not cyclo-oxygenase products. 28 references, 4 figures, 2 tables.

• Research Organization: Population Council, New York, NY
• OSTI ID: 6150566
• Journal Information: Life Sci.; (United States), Vol. 41:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
LIPASE
PHORBOL ESTERS
BIOLOGICAL EFFECTS
TESTOSTERONE
SECRETION
AMP
BIOLOGICAL PATHWAYS
EXPERIMENTAL DATA
INHIBITION
METABOLITES
RATS
RESPONSE MODIFYING FACTORS
ANDROGENS
ANDROSTANES
ANIMALS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARCINOGENS
DATA
ENZYMES
ESTERASES
ESTERS
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
INFORMATION
KETONES
KINETICS
MAMMALS
MONOCARBOXYLIC ACIDS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology