Oxygen radicals stimulate guinea pig gallbladder glycoprotein secretion in vitro

Journal Article · Sun Nov 01 04:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:7117866

Hale, W B; Turner, B; LaMont, J T ^[1]

---

1. Boston Univ. School of Medicine, MA (USA)

In several animal models of cholelithiasis, and in humans with gallstones, hypersecretion of gallbladder mucin is observed. This study was undertaken to determine the effect of oxygen radicals on guinea pig gallbladder glycoprotein secretion in organ culture. Mucosal explants were incubated with ({sup 3}H)glucosamine hydrochloride to label glycoproteins, then exposed to oxygen radicals generated by chelated ferric iron and ascorbic acid. Marked stimulation of glycoprotein release was observed after a 30-min exposure to the oxygen radical-generating system, and the effect was inhibited by mannitol. The stimulatory effect of hydroxyl radical was not accompanied by leakage of intracellular lactate dehydrogenase. Parallel experiments with human granulocytes activated with f-Met-Leu-Phe and coincubated with gallbladder explants revealed similar results. These results indicate that oxygen radicals, especially the hydroxyl radical (OH), are capable of stimulating rapid release of mucous-type glycoproteins from gallbladder epithelium.

• OSTI ID: 7117866
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
BILIARY TRACT
BIOLOGICAL EFFECTS
CARBOHYDRATES
DIGESTIVE SYSTEM
ELEMENTS
GLUCOSAMINE
GLYCOPROTEINS
HEXOSAMINES
HEXOSES
HYDROGEN COMPOUNDS
HYDROXYL RADICALS
MAMMALS
MAN
MONOSACCHARIDES
NONMETALS
ORGANIC COMPOUNDS
OXYGEN
PHYSIOLOGY
PRIMATES
PROTEINS
RADICALS
SACCHARIDES
SECRETION
SUPEROXIDE RADICALS
TRITIUM COMPOUNDS
VERTEBRATES