Reduction of nonselective adsorption of proteins by hydrophilization of microfiltration membranes by radiation-induced grafting

Journal Article · · Biotechnology Progress; (United States)

DOI:https://doi.org/10.1021/bp00025a013· OSTI ID:7112920

Kim, M; Kojima, Jun; Saito, Kyoichi; Furusaki, Shintaro ^[1]; Sugo, Takanobu ^[2]

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1. Univ. of Tokyo (Japan)
2. Japan Atomic Energy Research Inst., Takasaki (Japan)

Alcoholic hydroxyl groups were introduced into a polyethylene microfiltration (MF) membrane by radiation-induced graft polymerization of 2-hydroxyethyl methacrylate (HEMA), vinyl acetate (VAc), and glycidyl methacrylate (GMA). Subsequently, the VAc- and GMA-grafted membranes were quantitatively hydrolyzed into monool (single hydroxyl) and diol groups, respectively. The pure water flux of the modified membrane and the saturation capacity of bovine [gamma]-globulin onto the membrane were determined as a function of alcoholic hydroxyl group density. The threshold value for hydrophilization ranged between 5 and 7 mol of alcoholic hydroxyl group per kilogram of original MF membrane. Masking of the polyethylene surface with grafted polymer branches containing the diol groups was effective because approximately 70% of the pure water flux of the original MF membrane was maintained. Irrespective of the pore diameter of the original MF membrane, saturation capacities on the modified membrane correlated well with the diol group density. Saturation capacities of bovine [gamma]-globulin and bovine serum albumin were reduced to 1 mg/m[sup 2] of the membrane. In addition, the binding interaction changed from irreversible to reversible. 15 refs., 9 figs., 1 tab.

• OSTI ID: 7112920
• Journal Information: Biotechnology Progress; (United States), Journal Name: Biotechnology Progress; (United States) Vol. 10:1; ISSN 8756-7938; ISSN BIPRET
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201 -- Chemical & Physicochemical Properties
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADSORPTION
ALBUMINS
BLOOD SERUM
CARBOXYLIC ACID ESTERS
CHEMICAL REACTIONS
COLLOIDS
DECOMPOSITION
DISPERSIONS
ESTERS
FILTRATION
GELS
GLOBULINS
GRAFT POLYMERS
HYDROLYSIS
HYDROPHYLIC POLYMERS
HYDROXYLATION
LYSIS
MEMBRANE TRANSPORT
MEMBRANES
METHACRYLIC ACID ESTERS
MONOMERS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
POLYETHYLENES
POLYMERIZATION
POLYMERS
POLYOLEFINS
PROCESSING
PROTEINS
RADIATION EFFECTS
SEPARATION PROCESSES
SOLVOLYSIS
SORPTION
VINYL MONOMERS