Radioimmunoassay for 3,3',5'-L-triiodothyronine (reverse T/sub 3/): assessment of thyroid gland content and serum measurements in conditions of normal and altered thyroidal economy and following administration of thyrotropin releasing hormone (TRH) and thyrotropin (TSH). [/sup 125/I tracer technique]

Journal Article · Thu Mar 31 23:00:00 EST 1977 · J. Clin. Endocrinol. Metab.; (United States)

DOI:https://doi.org/10.1210/jcem-44-4-660· OSTI ID:7110221

Burman, K D; Dimon, R C; Wright, F D; Earll, J M; Bruton, J; Wartofskii, L

The present report describes the development of a radioimmunoassay for 3,3',5'-L-triiodothyronine (reverse T/sub 3/) which is performed on unextracted serum. Utilizing this radioimmunoassay, 21 normal subjects had a mean (+-SD) serum reverse T/sub 3/ level of 60 +- 12 ng/100 ml, 17 of 19 hyperthyroid patients had elevated serum reverse T/sub 3/ levels, and 10 of 11 hypothyroid subjects had decreased serum reverse T/sub 3/ concentrations. Thyroidal secretion of reverse T/sub 3/ was assessed by measurements in samples obtained from the internal carotid artery and jugular vein of sheep following the administration of thyrotropin releasing hormone (TRH) or bovine thyrotropin (TSH). Reverse T/sub 3/ levels were increased 45-60 min after TRH administration, but TSH administration produced inconsistent alterations in reverse T/sub 3/, although 18 of 27 samples obtained after TSH injection were higher than their average respective baseline concentration and the mean peak reverse T/sub 3/ level was 14 percent higher than baseline. Following TRH administration to 10 normal human subjects, mean serum reverse T/sub 3/ levels significantly increased from 53.6 ng/100 ml to 56.3 ng/100 ml (P less than .05). The thyroid gland content of reverse T/sub 3/ in human autopsy material was 6.5 +- 1.5 ..mu..g/g tissue. Both pregnancy and estrogen administration were associated with increases in serum reverse T/sub 3/ concentrations presumably because of their ability to augment thyroxine binding globulin synthesis.

• Research Organization: Walter Reed Army Medical Center, Washington, DC
• OSTI ID: 7110221
• Journal Information: J. Clin. Endocrinol. Metab.; (United States), Journal Name: J. Clin. Endocrinol. Metab.; (United States) Vol. 44:4; ISSN JCEMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BLOOD SERUM
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GLANDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SECRETION
THYROID
THYROID HORMONES
TRACER TECHNIQUES
TRH
TRIIODOTHYRONINE
TSH