mRNA-mediated transfer of genetic information in goldfish eggs

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7108573

Niu, L C; Xue, G X; Yang, J; Niu, M C

Rabbit globin-mRNA-injected eggs developed red blood cells containing rabbit globin. The mechanism by which the mRNA message was expressed was first suggested by the finding of mRNA transcript (cDNA) in enucleated eggs injected with rabbit globin mRNA. With Southern blotting, this cDNA was hybridized with P/sup 32/-..beta../sub 1/cDNA plasmid (P/sup 32/-pRcB/sub 1/). They found complementary DNA sequence but not in the DNA isolated from uninjected eggs and P/sup 32/pRcB/sub 1/. This result showed the injected-mRNA primed synthesis of rabbit ..beta../sub 1/ cDNA (..beta../sub 1/-globin gene). The fate of the globin cDNA was traced by (1) hybridization of the DNAs from developing stages of the mRNA-injected-and control eggs with P/sup 32/-pRc..beta../sub 1/. They found complementary DNA sequence in the former but not in the latter, thus showing the incorporation of globin cDNA into the DNA of mRNA injected fish, and (2) autoradiograms from sections of the globin H/sup 3/-cDNA (in vitro synthesized)-injected blastula with and without colchicine treatment (5..mu..g/ml). Silver grains were found in areas of nuclei of the control and the colchine treated blastula had them on metaphase chromosomes. Injected albumin mRNA was found to act in accord with the injected-globin-mRNA. The fate of these two mRNA transcripts leads to the proposal that the mRNA transcript in cleaving eggs may be responsible for differential gene activation during early embryogenesis.

• Research Organization: Temple Univ., Philadelphia PA
• OSTI ID: 7108573
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMALS
AQUATIC ORGANISMS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CELL NUCLEI
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
EGGS
FISHES
GLOBIN
HYBRIDIZATION
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RABBITS
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRITIUM COMPOUNDS
VERTEBRATES