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Retroviral-mediated transfer of genomic globin genes leads to regulated production of RNA and protein

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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A high-titer amphotropic retroviral vector containing the neomycin resistance gene and a hybrid ..gamma..-..beta.. genomic human globin gene has been constructed. Mouse erythroleukemia cells infected with this virus were found to contain the full transcriptional unit of the transferred human globin gene by Southern blot analysis. These cells contain normally initiated, spliced, and terminated human globin mRNA. The human globin mRNA level increased 5- to 10-fold upon induction of the mouse erythroleukemia cells. Human globin chains were produced but only in a fraction of the cells as detected by immunofluorescent staining. A similar retrovirus containing a human ..beta..-globin gene was used to transduce mouse erythroleukemia cells resulting in much higher levels of human globin synthesis than detected in mouse erythroleukemia cells transduced with the ..gamma..-..beta.. globin virus.

Research Organization:
National Institutes of Health, Bethesda, MD
OSTI ID:
6025262
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:8; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
ESTERASES
GENES
GENETIC MAPPING
GLOBIN
HEMIC DISEASES
HYDROLASES
ISOTOPES
LABELLED COMPOUNDS
LEUCINE
LEUKEMIA
LIGHT NUCLEI
MAMMALS
MAN
MAPPING
MESSENGER-RNA
MICE
MICROORGANISMS
NEOPLASMS
NUCLEASES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SYNTHESIS
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES
VIRUSES