Collagen crosslink location: a molecular marker for fibrosis in lungs of rats with experimental silicosis

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7107750

Gerriets, J E; Reiser, K M; Last, J A

Collagen content is increased in lungs of animals with experimental silicosis. They hypothesize that the collagen deposited in such fibrotic lungs differs structurally from normal lung collagen. Silicotic lung collagen shows an increase in lysine hydroxylation. In addition, the ratio of the difunctional crosslinks DHLNL (dihydroxylysinonorleucine) to HLNL (hydroxylysinonorleucine) is sharply elevated compared to that in control lungs. The peptide ..cap alpha..1(I)CB7 x ..cap alpha..2(I)CB1 crosslinked by HLNL was demonstrated in NaB/sup 3/H/sub 4/-reduced, CNBr-digested collagen from rat tail tendon by peptide purification, followed by periodate oxidation and amino acid analysis. Further structural analysis of this peptide was obtained by digestion of the crosslinked peptide with trypsin and purification of the tryptic peptide containing this crosslink followed by amino acid analysis. They then examined the analogous collagenous peptide in normal and silicotic lungs and analyzed the crosslink it contained. They observed that DHLNL was present at specific sites previously containing HLNL; that is, the collagen in fibrotic lungs is altered at specific sites by post-translational modification of a lysine residue by hydroxylation in a predictable way. They conclude that such unusual hydroxylation of a specific lysine residue in the ..cap alpha..2 chain provides a molecular marker for fibrotic lung collagen.

• Research Organization: Univ. of California, Davis
• OSTI ID: 7107750
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
BIOLOGICAL LOCALIZATION
BIOLOGICAL MARKERS
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COLLAGEN
CROSS-LINKING
DISEASES
FIBROSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
LUNGS
MAMMALS
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PEPTIDES
PNEUMOCONIOSES
POLYMERIZATION
PROTEINS
RATS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
SCLEROPROTEINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES