Identification and characterization of the surface proteins of Clostridium difficile
Several clostridial proteins were detected on the clostridial cell surface by sensitive radioiodination techniques. Two major proteins and six minor proteins comprised the radioiodinated proteins on the clostridial cell surface. Cellular fractionation of surface radiolabeled C. difficile determined that the radioiodinated proteins were found in the cell wall fraction of C. difficile and surprisingly were also present in the clostridial membrane. Furthermore, an interesting phenomenon of disulfide-crosslinking of the cell surface proteins of C. difficile was observed. Disulfide-linked protein complexes were found in both the membrane and cell wall fractions. In addition, the cell surface proteins of C. difficile were found to be released into the culture medium. In attempts to further characterize the clostridial proteins recombinant DNA techniques were employed. In addition, the role of the clostridial cell surface proteins in the interactions of C. difficile with human PMNs was also investigated.
- Research Organization:
- Indiana Univ., Bloomington, IN (USA)
- OSTI ID:
- 7105634
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BACTERIA
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CELL WALL
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
CLOSTRIDIUM
CROSS-LINKING
DNA
FRACTIONATION
FUNCTIONS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MICROORGANISMS
NEUTROPHILS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POLYMERIZATION
PRIMATES
PROTEINS
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES