Differences in the incorporation of bromodeoxyuridine by human lymphoblastoid cell lines

Journal Article · Fri Aug 01 00:00:00 EDT 1975 · Cell; (United States)

DOI:https://doi.org/10.1016/0092-8674(75)90057-4· OSTI ID:7103960

Henderson, E E; Strauss, B

Long term human lymphoblastoid lines differ in their ability to grow in medium containing bromodeoxyuridine (BrdU) and to incorporate analog into their DNA. Eight Burkitts' lymphoma cell lines divided at least twice in BrdU-containing medium and made DNA in which over 90 percent of the thymidine residues were substituted with analog in both strands. Three infectious mononucleosis-derived lines and 24 lines transformed in vitro were inhibited by BrdU after one cell division and made only hybrid DNA in which one strand was substituted with analog. One out of eight normal individuals from whom long term lines were prepared gave cell lines which divided at least twice in BrdU and gave DNA in which both strands were substituted with analog. It would appear that intrinsic cellular factors regulate the response to BrdU and that Burkitt's tumor lines are characterized by their ability to make stable doubly substituted DNA containing a high proportion of halogenated analog.

• Research Organization: Univ. of Chicago
• OSTI ID: 7103960
• Journal Information: Cell; (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BUDR
METABOLISM
DNA
LYMPHOCYTES
TUMOR CELLS
CELL DIVISION
LYMPHOMAS
THYMIDINE
ANIMAL CELLS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BROMOURACILS
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LEUKOCYTES
NEOPLASMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
URACILS
550500* - Metabolism