Horticultural characteristics of transgenic tobacco expressing the rolC gene from Agrobacterium rhizogenes

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · Journal of the American Society for Horticultural Science; (United States)

OSTI ID:7101596

Scorza, R; Zimmerman, T W; Cordts, J M; Footen, K J ^[1]; Ravelonandro, M ^[2]

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1. Dept. of Agriculture, Kearneysville, WV (United States)
2. Inst. National Recherche Agronomique, Villenave d'Ornon (France). Station de Pathologie Vegetale

Wisconsin 38 tobacco (Nicotiana tabacum L.) leaf discs were transformed with the disarmed Agrobacterium tumefaciens strain EHA 101 carrying the rolC gene from A. rhizogenes and NPT II and GUS genes. Shoots that regenerated on kanamycin-containing medium were confirmed as transgenic through GUS assays, polymerase chain reaction (PCR), Southern blot analyses, and transmission of the foreign genes through the sexual cycle. Transgenic plants were as short as half the height of control plants; were earlier flowering by up to 35 days; and had smaller leaves, shorter internodes, smaller seed capsules, fewer seeds, smaller flowers, and reduced pollen viability. The number of seed capsules, leaf number, and specific root length were similar between transgenic and control plants. Transgenic clones varied in the expression of the rolC-induced growth alterations as did the first generation of seedlings from these clones. Such differences suggested the potential for selecting for different levels of expression. Transformation with the rolC gene presents a potentially useful method of genetically modifying horticultural crops, particularly for flowering date, height, and leaf and flower size. Chemical names used: neomycin phosphotransferase (NPTII), [beta]-glucuronidase (GUS).

• OSTI ID: 7101596
• Journal Information: Journal of the American Society for Horticultural Science; (United States), Journal Name: Journal of the American Society for Horticultural Science; (United States) Vol. 119:5; ISSN JOSHB5; ISSN 0003-1062
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOTECHNOLOGY
CLONING
DNA HYBRIDIZATION
DNA-CLONING
GENE AMPLIFICATION
GENE REGULATION
GENETIC ENGINEERING
GROWTH
HYBRIDIZATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
NICOTIANA
PLANT BREEDING
PLANT GROWTH
PLANTS
POLYMERASE CHAIN REACTION