Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay

Journal Article · Wed Oct 10 00:00:00 EDT 1979 · J. Biol. Chem.; (United States)

OSTI ID:7098410

Sankaran, H; Deveney, C W; Goldfine, I D; Williams, J A

A modified method is described for the preparation of stable, high specific activity radioiodinated cholecystokinin (CCK) by its conjugation to /sup 125/I-Bolton Hunter reagent (/sup 125/I-BH). Purified radioiodinated CCK (/sup 125/I-BH-CCK) stimulated amylase release from isolated rat pancreatic acini with a potency identical to that of native CCK. Further, /sup 125/I-BH-CCK was highly immunoreactive and reacted with antisera directed toward both the NH/sub 2/- and COOH-terminal portions of the CCK molecule. Finally, /sup 125/I-BH-CCK bound to specific receptor sites on isolated pancreatic acini.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 7098410
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:19; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMYLASE
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BODY
CHEMICAL PREPARATION
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ENZYMES
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KININS
LABELLED COMPOUNDS
MAMMALS
NUCLEI
O-GLYCOSYL HYDROLASES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES